LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2198..2294) /label=attL2 /note="attL2 (c)" misc_signal complement(2309..2328) /note="T7 Promoter/priming site (c)" primer_bind 2336..2352 /note="M13 Reverse priming site" gene 2465..3274 /note="Kanamycin resistance gene" rep_origin 3395..4068 /note="pUC origin" PCR_frame 669..2198 /note="Reading Frame" /set="Set 1" PCR_primer 652..668 /primer="GTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC" /set="Set 1" /pair="Pair 1" /current=1 /original=ACAAGTTTGTACAAAAAAGCAGGCTCATGGAACAAGGACAGCTCTTCC PCR_primer complement(669..2218) /primer="AAAGCTGGGTTTAGCAGTGAATGGTCCG" /set="Set 1" /pair="Pair 1" /current=1 /original=ACCACTTTGTACAAGAAAGCTGGGTTTAGCAGTGAATGGTCCG PCR_product complement(652..2215) /pair="Pair 1" /set="Set 1" /current=1 /score=1.000 vector join(2206..4071,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 2176..2196 /note="TEV site" source 673..2175 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:2159 IMAGE:3542405" /tissue_type="Lymph, Burkitt lymphoma" /clone_lib="NIH_MGC_8" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 673..2175 /gene="PDIA3" /gene_synonym=ERp57 /gene_synonym=ERp60 /gene_synonym=ERp61 /gene_synonym=GRP57 /gene_synonym=HsT17083 /gene_synonym=P58 /gene_synonym=PI-PLC /db_xref="GeneID:2923" /db_xref="HGNC:4606" /db_xref="MIM:602046" CDS 673..2175 /dnas_title="protein disulfide isomerase family A, member 3" /gene="PDIA3" /gene_synonym=ERp57 /gene_synonym=ERp60 /gene_synonym=ERp61 /gene_synonym=GRP57 /gene_synonym=HsT17083 /gene_synonym=P58 /gene_synonym=PI-PLC /codon_start=1 /product="protein disulfide isomerase family A, member 3" /protein_id="AAH14433.1" /db_xref="GI:15680173" /db_xref="GeneID:2923" /db_xref="HGNC:4606" /db_xref="MIM:602046" /translation="MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGS AGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPT LKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFF DDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDK TVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGS NYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGE KFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNN ENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGF PTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL" misc_feature 673..2175 /note="PDIA3 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTCG CCatgcgcct ccgccgccta gcgctgttcc cgggtgtggc gctgcttctt 721 gccgcggccc gcctcgccgc tgcctccgac gtgctagaac tcacggacga caacttcgag 781 agtcgcatct ccgacacggg ctctgcgggc ctcatgctcg tcgagttctt cgccccctgg 841 tgtggacact gcaagagact tgcacctgag tatgaagctg cagctaccag attaaaagga 901 atagtcccat tagcaaaggt tgattgcact gccaacacta acacctgtaa taaatatgga 961 gtcagtggat atccaaccct gaagatattt agagatggtg aagaagcagg tgcttatgat 1021 ggacctagga ctgctgatgg aattgtcagc cacttgaaga agcaggcagg accagcttca 1081 gtgcctctca ggactgagga agaatttaag aaattcatta gtgataaaga tgcctctata 1141 gtaggttttt tcgatgattc attcagtgag gctcactccg agttcctaaa agcagccagc 1201 aacttgaggg ataactaccg atttgcacat acgaatgttg agtctctggt gaacgagtat 1261 gatgataatg gagagggtat catcttattt cgtccttcac atctcactaa caagtttgag 1321 gacaagactg tggcatatac agagcaaaaa atgaccagtg gcaaaattaa aaagtttatc 1381 caggaaaaca tttttggtat ctgccctcac atgacagaag acaataaaga tttgatacag 1441 ggcaaggact tacttattgc ttactatgat gtggactatg aaaagaacgc taaaggttcc 1501 aactactgga gaaacagggt aatgatggtg gcaaagaaat tcctggatgc tgggcacaaa 1561 ctcaactttg ctgtagctag ccgcaaaacc tttagccatg aactttctga ttttggcttg 1621 gagagcactg ctggagagat tcctgttgtt gctatcagaa ctgctaaagg agagaagttt 1681 gtcatgcagg aggagttctc gcgtgatggg aaggctctgg agaggttcct gcaggattac 1741 tttgatggca atctgaagag atacctgaag tctgaaccta tcccagagag caatgatggg 1801 cctgtgaagg tagtggtagc agagaatttt gatgaaatag tgaataatga aaataaagat 1861 gtgctgattg aattttatgc cccttggtgt ggtcactgta agaacctgga gcccaagtat 1921 aaagaacttg gcgagaagct cagcaaagac ccaaatatcg tcatagccaa gatggatgcc 1981 acagccaatg atgtgccttc tccatatgaa gtcagaggtt ttcctaccat atacttctct 2041 ccagccaaca agaagctaaa tccaaagaaa tatgaaggtg gccgtgaatt aagtgatttt 2101 attagctatc tacaaagaga agctacaaac ccccctgtaa ttcaagaaga aaaacccaag 2161 aagaagaaga aggcagaaaa cttgtacttt caaggctCCA GCTTTCTTGT ACAAAGTTGG 2221 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 2281 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 2341 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 2401 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2461 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2521 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 2581 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 2641 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2701 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2761 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2821 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2881 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 2941 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 3001 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 3061 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 3121 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 3181 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 3241 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 3301 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 3361 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3421 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3481 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3541 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3601 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3661 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3721 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3781 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3841 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3901 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 3961 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 4021 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //